Research Article

Xenobiotic Induced Expression of Epsilon Glutathione S – Transferases Gste2 In Laboratory Strains of Anopheles Arabiensis  

A.M. Yayo1,2 , A. Ado1 , M. Safiyanu3 , B.R. Muhammad4 , F.I. Sambo5 , A. Abubakar2 , J. Hemingway6
1 Centre for Infectious Diseases Research, Bayero University, Kano, Nigeria
2 Dept. of Medical Parasitology and Microbiology, Bayero University, Kano, Nigeria
3 Dept of Biochemistry, Yusufu Maitama Sule, University Kano, Nigeria
4 Dept of Veterinary Parasitology and Entomology, University of Abuja, Abuja, Nigeria
5 Dept of Biological Sciences, Yusufu Maitama Sule, University Kano, Kano, Nigeria
6 Vector Biology Research Group, Liverpool School of Tropical Medicine, England
Author    Correspondence author
Molecular Entomology, 2019, Vol. 10, No. 1   
Received: 13 Nov., 2019    Accepted: 30 Nov., 2019    Published: 20 Dec., 2019
© 2019 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

Over expression of GSTe2 has been associated with resistance to DDT in An. arabiensis. The expression of the gene was induced by exposing the fourth instar larvae obtained from KGB and MAT strains to xenobiotic inducers. Transcripts levels of GSTe2 in cDNAs of the larvae from each strain was measured by quantitative PCR at various time points post exposure to DDT, permethrin and hydrogen peroxide. Statistical analysis was used to test for differences and compare the expression of the gene between treatments and mosquito strains. The geometric mean expression levels of GSTe2 induced at one hour by DDT in the KGB was 1.028 (CI 0.901~1.172; p < 100) compared to 0.587 (CI 0.475~0.724; p = 0.025) in MAT strain. Higher induction of GSTe2 in KGB was caused by DDT and hydrogen peroxide and with permethrin and DDT in MAT strain. Further research is needed to understand the molecular mechanisms regulating response of An. arabiensis to xenobitics.

Keywords
Xenobiotic; Expression; Glutathione S-Transferases Gste2; Anopheles arabiensis
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Molecular Entomology
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. A.M. Yayo
. A. Ado
. M. Safiyanu
. B.R. Muhammad
. F.I. Sambo
. A. Abubakar
. J. Hemingway
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